Methodological aspects of increasing the intensity of callusogenesis and organogenesis of Linum usitatissimum L. in vitro

Aim. Improving methods for increasing the efficiency of obtaining callus cultures and somaclones flax (Linum usitatissimum L.) in vitro. Methods. Hypocotyl segments were cultured on Murashige Skoog nutrient medium supplemented with sucrose (30 g/l) phytohormones at various concentrations. Other conditions: photoperiod 16 hours, illuminance 2500 lx, relative humidity 60–80%, air temperature 22–24°C. Results. The ability to form somatic embryogenesis depends phytohormonal composition medium, size explants distance between them. Conclusions. For intensive formation vitro, optimal concentrations BAP (mg/l) can be expressed as 1.0 ≤ 1.75; NAA (0.05 mg/l) 0.5 2.0; concentration (1.0 0.025 0.150; IAA 0.05 0.50. Addition mg/l GA3 is effective. It use hypocotyl 3–6 mm long place them a 1.5–2.5 cm from each other. Organogenicity significantly reduced process subculturing.